Protein Structural Biology Online Course

This webpage includes freely downloadable videos and presentations based on the book: 

 Introduction to proteins: structure, function, and motion  

Please acknowledge the book when using the materials .


Chapter 1


  • What are macromolecules?

  • Proteins' biological roles

  • The structural base of protein function

  • Non-covalent interactions

Chapter 4

Energetics & Stability

  • Proteins are marginally stable

  • Thermodynamics of protein structure stability

  • Physical forces and effects that stabilize or destabilize the folded structure

  • Protein denaturation and adaptation to extreme environments

  • Protein engineering for increased stability

Chapter 8

Protein-ligand interactions

  • Biological importance

  • Binding affinity: measurements, calculations, and thermodynamics

  • Binding specificity: models, interactions, binding promiscuity

  • Case study: cholinesterase inhibition by toxins and drugs

  • Protein-ligand interactions in drug design

Chapter 2

Protein structure

  • Amino acids properties

  • The peptide bond

  • Secondary structures

  • Tertiary structure of globular proteins

  • Structural evolution

  • Quaternary structure

  • Post-translational modifications

  • Fibrous proteins

Chapter 5


  • The importance of protein dynamics

  • Types of motions in proteins and their biological roles

  • Theoretical models of protein dynamics

  • Thermodynamic and kinetic effects

  • External effects on protein dynamics (e.g. ligand binding)

  • Protein folding and misfolding

Chapter 3

Structure Prediction

  • Why predict protein structure?

  • General apporaches for structure prediction

  • The physical approach: explicit and mean-field models

  • The comparative approach: homology modeling and fold recognition

  • Integrative methods

  • Experimentally guided prediction

  • Prediction based on co-evolution

Chapter 7

Membrane proteins

  • Biological roles

  • The lipid bilayer environment

  • Integral membrane proteins: structure, composition & energetics

  • Peripheral membrane proteins

  • Effects of membrane properties and lipids on bound proteins

  • G protein-coupled receptors: structure-function relationships


Chapters 6 and 9 are in preparation and will be added later on

Tel-Aviv University course version (videos by lesson)

Last updated: Apr 29 2021